New Test Guidelines Available for Eye Safety Testing
The Organisation for Economic Co-operation and Development (OECD) has officially adopted two test guidelines for identification of substances with the potential to cause eyeinjury. One of these, an updated test guideline for the traditional rabbit eye test, incorporates specific procedures to avoid or minimize animal pain anddistress when it necessary to use animals to identify substances with the potential to cause eye injuries. The other test guideline provides a new method to identify substances that may cause serious eye injuries without using animals.
The new test guidelines are available on the NICEATM-ICCVAM website:
- OECD Test Guideline 405: «Acute Eye Irritation/Corrosion» is available at http://iccvam.niehs.nih.gov/SuppDocs/FedDocs/OECD/OECD-TG405-2012-508.pdf
- OECD Test Guideline 460: «Fluorescein Leakage Test Method for Identifying Ocular Corrosives andSevere Irritants» is available at http://iccvam.niehs.nih.gov/SuppDocs/FedDocs/OECD/OECD-TG460-508.pdf
Updated Test Guideline 405 Incorporates ICCVAM Recommendations
The updated Test Guideline 405 incorporates recommendations made by the Interagency Coordinating Committee on the Validation of Alternative Toxicological Methods (ICCVAM) to U.S. Federal agencies in 2010. After an evaluation of the use of anesthetics, analgesics, and humane endpoints in eye safety testing, ICCVAM recommended that pain management procedures should always be used when it is determined necessary to use the rabbit eye test for regulatory safety assessments.
The ICCVAM recommendations were accepted or endorsed by U.S. Federal agencies in 2011. The adoption of the updated OECD test guideline 405 means that these procedures may now be used in the 34 member countries of the OECD, refining animal use for eye safety testing in many more countries.
ICATM Partner Evaluation Leads to Adoption of Test Guideline 460
The fluorescein leakage (FL) test method can be used to identify ocular corrosives and severe irritants: substances that can cause severe, permanent eye injuries. While it is not a complete replacement for the rabbit eye test, the FL test method can be used as an initial step in a testing strategy to identify water-soluble substances and mixtures that are potential corrosives or severe irritants.
The FL test method was adopted by OECD after being evaluated by the European Union Reference Laboratory for Alternatives to Animal Testing. NICEATM and ICCVAM contributed to the evaluation of the FL test as members of the International Cooperation on Alternative Test Methods (ICATM).